Nanobiotechnology

Biography

Biography: John M Tomich

Abstract

Branched Amphipathic Peptide Capsules (BAPCs) are biologically derived, bilayer delimited, nano-vesicles capable of being coated by or encapsulating a wide variety of solutes. The vesicles and their cargos are readily taken up by cells and become localized in the peri-nuclear region of cells. When BAPCs are mixed with DNA, the BAPCs act as cationic nucleation centers around which DNA winds. The BAPCs-DNA nanoparticles are capable of delivering plasmid DNA and RNAs in vivo and in vitro yielding high transfection rates and minimal cytotoxicity. In this study, we inhibited expression of two insect genes, BiP and Armet, through the ingestion of dsRNA complexed with BAPCs. The dsRNA-BAPCs complexes were added to the diets of two insect species from two Orders: Acyrthosiphon pisum and Tribolium castaneum. The gene transcripts tested (BiP and Armet) are part of the unfolded protein response (UPR) and suppressing their translation resulted in lethality. For Acyrthosiphon pisum, ingestion of <10 ng of BiP-dsRNA associated with BAPCs led to the premature death of the aphids (t_1/2=4 - 5 days) compared to ingestion of the same amounts of free BiP-dsRNA (t_1/2=11-12 days). Tribolium was effectively killed by ingestion (by larvae only) using a combination of BiP-dsRNA and Armet-dsRNA complexed with BAPCs (75% of the subjects, n=30) died as larvae or during eclosion (the emergence of adults from pupae). Feeding the two dsRNA alone resulted in fewer deaths (30% with n=30). These results show that complexation of dsRNA with BAPCs greatly enhances the oral delivery of dsRNA over dsRNA alone in the diet. This approach provides a simpler method of delivering dsRNA compared to microinjection for studying in vivo protein function and for developing novel strategies for pest management.